>>> Chimie Bioorganique et Systèmes Amphiphiles (IBMM - UMR 5247)

Structure(s) de rattachement

Département Chimie
UFR-ip Sciences, Technologies, Santé

Rattachement(s) externe(s)

Institut des Biomolécules Max Mousseron (IBMM)

Formations proposées

CHIMIE

MASTER CHIMIE DU VIVANT
CHIMIE
PHYSIQUE-CHIMIE TRONC COMMUN
SVT - CHIMIE DU VIVANT TRONC COMMUN
CHIMIE BIOLOGIQUE
M1 : CHIMIE MOLECULAIRE DU VIVANT

Reconnaissance

Unité mixte de recherche

Direction scientifique

Chimie

Discipline de recherche

Les activités de recherche de l’équipe CBSA se positionnent aux interfaces de la chimie, de la biologie et de la physique autour de trois thématiques principales visant à développer : (1) des outils moléculaires amphiphiles utilisables pour la caractérisation des protéines membranaires, le dépôt contrôlé de nanoparticules, la mise au point d'agents de contraste ultrasonore ; (2) des vecteurs de type dendrimère ou oligomère pour le transport ciblé d’actifs à visée anti-inflammatoire, anticancéreuse et/ou anti-angiogénique; (3) de nouveaux anti-oxydants d’intérêt thérapeutique ainsi que l’étude de leurs propriétés anti-oxydantes en milieu chimique et biologique.

Nos activités de recherche peuvent trouver des applications dans des domaines aussi variés que le monde médical, biomédical, vétérinaire, agroalimentaire ou encore dans la fabrication de nouveaux transistors.

Adresse

33 rue Louis Pasteur 84000 Avignon

Responsable(s)

Prof. Ange Polidori 

Composition

Prof. A. Polidori (Directeur du laboratoire) - email

Dr. C. Pepin (Maitre de Conférences - HDR) - email

Dr. G. Durand (Directeur du département de chimie - Maitre de Conférences - HDR) - email

Dr. P. Guillet (Maitre de Conférences) - email

Dr. F. Bonneté (Chargé de recherche CNRS - HDR) - email

Dr. S. Desgranges (Post-doc) - email

S. Raynal (Technicien) - email

M. Rosselin (PhD student) - email

R. Gomez (PhD student) - email

Equipes

Pages web des membres

Pepin Christine
Durand Grégory
Guillet Pierre
Bonneté Françoise

Axes de recherche

 

Élaboration de nouvelles structures moléculaires amphiphiles, macromoléculaires ou supra-moléculaires biocompatibles susceptibles de moduler la biodisponibilité d'un principe actif (protéine, gène, médicament ou biocide). Mise au point de nouveaux systèmes anti-inflammatoires et/ou anti-oxydants contre les maladies neurodégénératives (Sclérose en Plaques, maladie d’Alzheimer…). Mise au point de systèmes supramoléculaires pour la manipulation et le dépôt de nanoparticules.

Publications

2013

1. Barret, L.-A., Polidori, A., Bonneté, F., Bernard-Savary, P., and Jungas, C. (2013) A new High-Performance Thin Layer Chromatography-based assay of detergents and surfactants commonly used in membrane protein studies, Journal of Chromatography A 10.1016/j.chroma.2013.01.061.

2. Mugemana, C., Joset, A., Guillet, P., Appavou, M.-S., De Souza, N., Fustin, C.-A., Leyh, B., Gohy, J.-F. (2013) Metallo-Supramolecular Micellar Gels: A Structural Study Langmuir In press (la-2013-00164m)

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2012

3. Fotinou, C., Aittoniemi, J., de Wet, H., Polidori, A., Pucci, B., Sansom, M. S. P., Vénien-Bryan, C., and Ashcroft, F. M. (2012) Tetrameric structure of SUR2B revealed by electron microscopy of oriented single particles., FEBS J.

4. Bonneté F. Macromolecular crystallization controlled by colloidal interactions: the case of urate oxidase (2012) in Crystallization / Book 1 Intech publisher edited by Dr. Marcello R. B. Andreeta ISBN 979−953−307−624

5. Bonneté F. (invited) Colloidal Properties of Biomacromolecular Solutions Relevant to Crystal Growth and Crystallography C R Physique 10.1016/j.crhy.2012.12.005

6. Pingret, D., Durand, G., Fabiano-Tixier, A.-S., Rockenbauer, A., Ginies, C., and Chemat, F. (2012) Degradation of edible oil during food processing by ultrasound: electron paramagnetic resonance, physicochemical, and sensory appreciation., J. Agric. Food Chem. 60, 7761–7768.

7. Rolland, J., Guillet, P., Schumers, J.-M., Duhem, N., Préat, V., and Gohy, J.-F. (2012) Polyelectrolyte complex nanoparticles from chitosan and poly(acrylic acid) and Polystyrene- block-poly(acrylic acid), journal of polymer Science Part A-Polymer Chemistry 50, 4484–4493.

8. Bechara, C., Bolbach, G., Bazzaco, P., Sharma, K. S., Durand, G., Popot, J.-L., Zito, F., and Sagan, S. (2012) MALDI-TOF mass spectrometry analysis of amphipol-trapped membrane proteins., Anal. Chem. 84, 6128–6135.

9. Rahmeh, R., Damian, M., Cottet, M., Orcel, H., Mendre, C., Durroux, T., Sharma, K. S., Durand, G., Pucci, B., Trinquet, E., Zwier, J. M., Deupi, X., Bron, P., Banères, J.-L., Mouillac, B., and Granier, S. (2012) Structural insights into biased G protein-coupled receptor signaling revealed by fluorescence spectroscopy., P Natl Acad Sci Usa 109, 6733–6738.

10. Kyrychenko, A., Rodnin, M. V., Vargas-Uribe, M., Sharma, S. K., Durand, G., Pucci, B., Popot, J.-L., and Ladokhin, A. S. (2012) Folding of diphtheria toxin T-domain in the presence of amphipols and fluorinated surfactants: Toward thermodynamic measurements of membrane protein folding., Biochim. Biophys. Acta 1818, 1006–1012.

11. Sharma, K. S., Durand, G., Gabel, F., Bazzacco, P., Le Bon, C., Billon-Denis, E., Catoire, L. J., Popot, J.-L., Ebel, C., and Pucci, B. (2012) Non-ionic amphiphilic homopolymers: synthesis, solution properties, and biochemical validation., Langmuir 28, 4625–4639.

12. Blesneac, I., Ravaud, S., Juillan-Binard, C., Barret, L.-A., Zoonens, M., Polidori, A., Miroux, B., Pucci, B., and Pebay-Peyroula, E. (2012) Production of UCP1 a membrane protein from the inner mitochondrial membrane using the cell free expression system in the presence of a fluorinated surfactant., Biochim. Biophys. Acta 1818, 798–805.

13. Karlik, S. J., Roscoe, W. A., Patinote, C., and Contino-Pépin, C. (2012) Targeting vascular changes in lesions in multiple sclerosis and experimental autoimmune encephalomyelitis., Cent Nerv Syst Agents Med Chem 12, 7–14.

14. Bazzacco, P., Billon-Denis, E., Sharma, K. S., Catoire, L. J., Mary, S., Le Bon, C., Point, E., Banères, J.-L., Durand, G., Zito, F., Pucci, B., and Popot, J.-L. (2012) Nonionic homopolymeric amphipols: application to membrane protein folding, cell-free synthesis, and solution nuclear magnetic resonance., Biochemistry 51, 1416–1430.

15. Duhem, N., Rolland, J., Riva, R., Guillet, P., Schumers, J.-M., Jerome, C., Gohy, J.-F., and Préat, V. (2012) Tocol modified glycol chitosan for the oral delivery of poorly soluble drugs, International Journal of Pharmaceutics 423, 452–460.

16. Choteau, F., Tuccio, B., Villamena, F. A., Charles, L., Pucci, B., and Durand, G. (2012) Synthesis of tris-hydroxymethyl-based nitrone derivatives with highly reactive nitronyl carbon., J Org Chem 77, 938–948.

17. Brassinne, J., Mugemana, C., Guillet, P., Bertrand, O., Auhl, D., Bailly, C., Fustin, C.-A., and Gohy, J.-F. (2012) Tuning micellar morphology and rheological behaviour of metallo-supramolecular micellar gels, Soft Matter 8, 4499.

18. Dauvergne, J., Bendjeriou, A., Bonneté, F., Kohlbrecher, J., Pucci, B., Barret, L.-A., and Polidori, A. (2012) Tripod facial surfactants with benzene as the central core: design, synthesis and self-assembly study, New J Chem 36, 1170.

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2011

19. Abla, M., Durand, G., and Pucci, B. (2011) Propyl-ended hemifluorinated surfactants: synthesis and self-assembling properties., J Org Chem 76, 2084–2093.

20. Hovers, J., Potschies, M., Polidori, A., Pucci, B., Raynal, S., Bonneté, F., Serrano-Vega, M. J., Tate, C. G., Picot, D., Pierre, Y., Popot, J.-L., Nehmé, R., Bidet, M., Mus-Veteau, I., Busskamp, H., Jung, K.-H., Marx, A., Timmins, P. A., and Welte, W. (2011) A class of mild surfactants that keep integral membrane proteins water-soluble for functional studies and crystallization., Mol. Membr. Biol. 28, 171–181.

21. Mugemana, C., Guillet, P., Fustin, C.-A., and Gohy, J.-F. (2011) Metallo-supramolecular block copolymer micelles: recent achievements, Soft Matter 7, 3673–3678.

22. Can, A., Hoeppener, S., Guillet, P., Gohy, J.-F., Hoogenboom, R., and Schubert, U. S. (2011) Upper Critical Solution Temperature Switchable Micelles Based on Polystyrene-block-poly(methyl acrylate) Block Copolymers, journal of polymer Science Part A-Polymer Chemistry 49, 3681–3687.

23. Giffard, M; Ferte, N; Ragot, F; El Hajji, M; Castro, B; Bonneté, F Urate Oxidase Purification by Salting-in Crystallization: Towards an Alternative to Chromatography (2011) PlosOne 6(5), e19013.

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2010

24. Choteau, F., Durand, G., Ranchon-Cole, I., Cercy, C., and Pucci, B. (2010) Cholesterol-based α-phenyl-N-tert-butyl nitrone derivatives as antioxidants against light-induced retinal degeneration., Bioorg Med Chem Lett 20, 7405–7409.

25. Contino-Pépin, C., Parat, A., Patinote, C., Roscoe, W. A., Karlik, S. J., and Pucci, B. (2010) Thalidomide derivatives for the treatment of neuroinflammation., ChemMedChem 5, 2057–2064.

26. Durand, G., Poeggeler, B., Ortial, S., Polidori, A., Villamena, F. A., Böker, J., Hardeland, R., Pappolla, M. A., and Pucci, B. (2010) Amphiphilic amide nitrones: a new class of protective agents acting as modifiers of mitochondrial metabolism., J Med Chem 53, 4849–4861.

27. Nehmé, R., Joubert, O., Bidet, M., Lacombe, B., Polidori, A., Pucci, B., and Mus-Veteau, I. (2010) Stability study of the human G-protein coupled receptor, Smoothened., Biochim. Biophys. Acta 1798, 1100–1110.

28. Mugemana, C., Guillet, P., Hoeppener, S., Schubert, U. S., Fustin, C.-A., and Gohy, J.-F. (2010) Metallo-supramolecular diblock copolymers based on heteroleptic cobalt(III) and nickel(II) bis-terpyridine complexes., Chem Commun 46, 1296–1298.

29. Vielzeuf D, Floquet N, Chatain D, Bonneté F, Ferry D, Garrabou J, Stolper E M. (2010) Multilevel Modular Mesocrystalline Organization in Red Coral J Am Min, 95, 242-248.

30. Girard E, Marchal S, Perez J, Finet S, Kahn R, Fourme R, Marassio G, Dhaussy AC, Prangé T, Giffard M, Dulin F, Bonneté F, Lange R, Abraini J, Mezouar M, and Colloc’h N. (2010) Structure-function perturbation and dissociation of tetrameric urate oxidase by high hydrostatic pressure. (2010) Biophysical Journal, 98, 10, 2365-2373.

31. Collings I, Watier Y, Giffard M, Dagogo S, Kahn R, Bonneté F, Wright J P.. Fitch A N and Margiolaki I (2010) Polymorphism of microcrystalline urate oxidase from Aspergillus flavus Acta Cryst D, 66, 539-548.

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2009

32. Bazzacco, P., Sharma, K. S., Durand, G., Giusti, F., Ebel, C., Popot, J.-L., and Pucci, B. (2009) Trapping and stabilization of integral membrane proteins by hydrophobically grafted glucose-based telomers., Biomacromolecules 10, 3317–3326.

33. Richard, C., Chaumet-Riffaud, P., Belland, A., Parat, A., Contino-Pépin, C., Bessodes, M., Scherman, D., Pucci, B., and Mignet, N. (2009) Amphiphilic perfluoroalkyl carbohydrates as new tools for liver imaging., International Journal of Pharmaceutics 379, 301–308.

34. Durand, G., Prosak, R. A., Han, Y., Ortial, S., Rockenbauer, A., Pucci, B., and Villamena, F. A. (2009) Spin trapping and cytoprotective properties of fluorinated amphiphilic carrier conjugates of cyclic versus linear nitrones., Chem. Res. Toxicol. 22, 1570–1581.

35. Joubert, O., Nehmé, R., Fleury, D., De Rivoyre, M., Bidet, M., Polidori, A., Ruat, M., Pucci, B., Mollat, P., and Mus-Veteau, I. (2009) Functional studies of membrane-bound and purified human Hedgehog receptor Patched expressed in yeast., Biochim. Biophys. Acta 1788, 1813–1821.

36. Breyton, C., Gabel, F., Abla, M., Pierre, Y., Lebaupain, F., Durand, G., Popot, J.-L., Ebel, C., and Pucci, B. (2009) Micellar and biochemical properties of (hemi)fluorinated surfactants are controlled by the size of the polar head., Biophys. J. 97, 1077–1086.

37. Han, Y., Liu, Y., Rockenbauer, A., Zweier, J. L., Durand, G., and Villamena, F. A. (2009) Lipophilic beta-cyclodextrin cyclic-nitrone conjugate: synthesis and spin trapping studies., J Org Chem 74, 5369–5380.

38. Talbot, J.-C., Dautant, A., Polidori, A., Pucci, B., Cohen-Bouhacina, T., Maali, A., Salin, B., Brèthes, D., Velours, J., and Giraud, M.-F. (2009) Hydrogenated and fluorinated surfactants derived from Tris(hydroxymethyl)-acrylamidomethane allow the purification of a highly active yeast F1-F0 ATP-synthase with an enhanced stability., J. Bioenerg. Biomembr. 41, 349–360.

39. Vlad, A., Dutu, C. A., Guillet, P., Jedrasik, P., Fustin, C.-A., dervall, U. S., Gohy, J.-F., and Melinte, S. (2009) Highly ordered conjugated polymer nanoarchitectures with three-dimensional structural control., Nano Lett 9, 2838–2843.

40. Contino-Pépin, C., Parat, A., Périno, S., Lenoir, C., Vidal, M., Galons, H., Karlik, S., and Pucci, B. (2009) Preliminary biological evaluations of new thalidomide analogues for multiple sclerosis application., Bioorg Med Chem Lett 19, 878–881.

41. Posokhov, Y. O., Rodnin, M. V., Kyrychenko, A., Holt, A., Contino-Pépin, C., Pucci, B., Killian, J. A., and Ladokhin, A. S. (2009) Can We Measure the Thermodynamic Stability of Membrane Proteins in a Lipid Bilayer Environment?, Biophys. J. 96, 333a.

42. Bazzacco, P., Sharma, K. S., Durand, G., Giusti, F., Ebel, C., Popot, J.-L., and Pucci, B. (2009) SI-Trapping and Stabilization of Integral Membrane Proteins by Hydrophobically Grafted Glucose-Based Telomers, Biomacromolecules 10, 3317–3326.

43. Guillet, P., Mugemana, C., Stadler, F. J., Schubert, U. S., Fustin, C.-A., Bailly, C., and Gohy, J.-F. (2009) Connecting micelles by metallo-supramolecular interactions: towards stimuli responsive hierarchical materials, Soft Matter 5, 3409–3411.

44. Guillet, P., Fustin, C.-A., Wouters, D., Hoeppener, S., Schubert, U. S., and Gohy, J.-F. (2009) Amphiphilic brushes from metallo-supramolecular block copolymers, Soft Matter 5, 1460–1465.

45. Gohy, J.-F., Chiper, M., Guillet, P., Fustin, C.-A., Hoeppener, S., Winter, A., Hoogenboom, R., and Schubert, U. S. (2009) Self-organization of rod-coil tri- and tetra-arm star metallo-supramolecular block copolymers in selective solvents, Soft Matter 5, 2954–2961.

46. Han, Y., Liu, Y., Rockenbauer, A., Zweier, J. L., Durand, G., and Villamena, F. A. (2009) Lipophilic β-Cyclodextrin Cyclic−Nitrone Conjugate: Synthesis and Spin Trapping Studies, J. Org. Chem., 74, 5369–5380.

47. M. Giffard, V. Delfosse, G. Sciara, C. Mayer, C. Cambillau, M. El Hajji, B. Castro, F. Bonneté (2009) Surfactant Poloxamer 188 as a New Crystallizing Agent for Urate Oxidase Crystal Growth and Design, 9, 4199-4206.

48. E. Oksanen, M.P. Blakeley, F. Bonneté, M.T. Dauvergne, F. Dauvergne, M. Budayova-spano (2009) Large crystal growth by thermal control allows combined X-ray and neutron crystallographic studies to elucidate the protonation states in Aspergillus flavus urate oxidase Journal of the Royal Society Interface, 6, S599-S610.

Equipements

RMN 400 MHz multi-noyaux (Bruker)

Spectromètre de résonance paramagnétique électronique (Magnettech)

Spectromètres infra-rouge et UV/vis (Varian), spectrophotomètre (FluoroMax-2)

Tensiomètre (Kruss K 100), granulomètre (Malvern Nano S), balance de Langmuir

Electrophorèse capillaire (Iatroscan MK-5), polarimètre

CLHP analytique semi-préparative et préparative (Varian & Waters)

Combiflashs

SEC organique triple-détection (Waters-Malvern)

SEC aqueuse RI+MALS (Wyatt)

Micro-onde, ultrasons (cuves Bransons et Bioblock VibraCell 75-043)

Lyophilisateur (BioBlock Alpha 1-4LD)

Voltampérométrie cyclique (Princeton)

Centrifugeuse (ThermoScientific)

Coopérations internationales

Outils amphiphiles pour la protéomique
Dr Wolfram Welte, Fachbereich biologie, Universitat Konstanz, Konstanz, Germany.
Pr Alex Ladokhin, Kansas University, Medical center.
Pr Andrès Salvay, Universidad Nacional de la Plata, Argentine

Vectorisation d’agents anti-angiogéniques
Dr Stephen Karlik, MS researches department, Children’s Hospital, London, Ontario
Pr M. Touaibia, Laboratoire de chimie médicinale, Université de Moncton, Canada.
Pr Qian Peng, Department of Pathology, The Oslo University Hospital, Norway.
Pr. John McLarnon, Department of anaesthesiology, pharmacology and therapeutics. University of British Columbia, Vancouver – Canada
Pr. R. Roy, Laboratoire de Chimie Médicinale. Chaire de recherche du Canada. Université du Québec à Montréal (UQAM), Montréal – Canada
 
Nouveaux agents antiradicalaires
Pr F. Villamena, Davis Heart and Lung Research Institute, Ohio S. Univ, Columbus,
Pr Antal Rockenbauer, Université de Budapest, Hongrie.

Collaborations universitaires

Outils amphiphiles pour la protéomique
Jean-Luc Popot, Institut de Biologie Physico-Chimique, UMR 7099, Paris
Eva Pebay-Peyroula, LPM, IBS, Grenoble
Bruno Ameduri, Institut Charles Gerhardt - UMR(CNRS) 5253, Montpellier
Jean Claude Talbot, IBGC UMR 5095, Bordeaux
Corinne Vives, IBS, LPM, Grenoble
Bruno Miroux, Biotram UPR CNRS 9078, Paris.
Jean Jacques Lacapère, INSERM U773, CRB3, Paris
Colette Jungas, CNRS UMR 6161, IBEB/SBVMe-SBVMe/LBC, Cadarache
Olivier Joubert, CEA/DSV/IBITEC-S/SIMOPRO/LTMN CEA Gif/Yvette
Marie-France Giraud, Institut de Biochimie et Génétique Cellulaire UMR 5095, Bordeaux
Christine Ebel, IBS, LBM, Grenoble
Valentin Gordelyi, IBS, LBM, Grenoble
Cécile Breyton, IBS, LBM, Grenoble
Jean-Louis Baneres, IBMM, Montpellier
Laurence Serres, LPM, IBS, Grenoble.
Isabelle Mus-Veteau, FRE CNRS 3094, Nice Sophia Antipolis

Agents de contraste ultrasonore nanométriques pour l’imagerie et le traitement médical
Nicolas Taulier, Laboratoire d’imagerie paramétrique, UMR 8550, Université Paris 6.

Organisation et dépôt de nanoparticules en film noir
Jean-Jacques Benattar, CNRS URA 2464) DSM/DRECAM/SPEC, CEA Saclay.

Nouveaux agents antiradicalaires
Isabelle Ranchon-Cole, Faculté de Pharmacie, Clermont Ferrand

Extraction sans solvants d’ions par flottation
Pierre Bauduin, Institut de Chimie Séparative de Marcoule (ICSM) UMR 5257-LIIC (Laboratoire des Ions aux Interfaces Corrosives)
Olivier Diat, Institut de Chimie Séparative de Marcoule (ICSM) UMR 5257-LIIC (Laboratoire des Ions aux Interfaces Corrosives)

Nanoparticules métalliques pegylées, modélisation du cheminement des virus dans les sols
Eric Michel, UMR EMMAH, Centre INRA d’Avignon.
Sophie Neveu, laboratoire PECSA UMR 7195, Université Paris 6.