>>> Chimie Bioorganique et Systèmes Amphiphiles (IBMM - UMR 5247)

Structure(s) de rattachement

Département Chimie
UFR-ip Sciences et Technologies

Rattachement(s) externe(s)

Institut des Biomolécules Max Mousseron (IBMM)

Formations proposées

CHIMIE
LICENCE CHIMIE
MASTER CHIMIE
CHIMIE
PHYSIQUE-CHIMIE TRONC COMMUN
SVT - CHIMIE DU VIVANT TRONC COMMUN
CHIMIE BIOLOGIQUE
M1 : CHIMIE MOLECULAIRE

Reconnaissance

Unité mixte de recherche

Direction scientifique

Chimie

Discipline de recherche

Les activités de recherche de l’équipe CBSA se positionnent aux interfaces de la chimie, de la biologie et de la physique autour de trois thématiques principales visant à développer : (1) des outils moléculaires amphiphiles utilisables pour la caractérisation des protéines membranaires, le dépôt contrôlé de nanoparticules, la mise au point d'agents de contraste ultrasonore ; (2) des vecteurs de type dendrimère ou oligomère pour le transport ciblé d’actifs à visée anti-inflammatoire, anticancéreuse et/ou anti-angiogénique; (3) de nouveaux anti-oxydants d’intérêt thérapeutique ainsi que l’étude de leurs propriétés anti-oxydantes en milieu chimique et biologique.

Nos activités de recherche peuvent trouver des applications dans des domaines aussi variés que le monde médical, biomédical, vétérinaire, agroalimentaire ou encore dans la fabrication de nouveaux transistors.

Adresse

33 rue Louis Pasteur 84000 Avignon

Responsable(s)

Prof. Ange Polidori 

Composition

Prof. A. Polidori (Directeur du laboratoire) - email

Dr. C. Pepin (Maitre de Conférences - HDR) - email

Dr. G. Durand (Maitre de Conférences - HDR) - email

Dr. P. Guillet (Maitre de Conférences) - email

Dr. F. Bonneté (Chargé de recherche CNRS) - email

Dr. F. Choteau-Mary (Post-doc) - email

Dr. M. Bosco (Post-doc) - email

S. Raynal (Technicien) - email

M. Rosselin (PhD student) - email

R. Gomez (PhD student) - email

Equipes

Pages web des membres

Pepin Christine
Durand Grégory
Guillet Pierre
Bonneté Françoise

Axes de recherche

 

Élaboration de nouvelles structures moléculaires amphiphiles, macromoléculaires ou supra-moléculaires biocompatibles susceptibles de moduler la biodisponibilité d'un principe actif (protéine, gène, médicament ou biocide). Mise au point de nouveaux systèmes anti-inflammatoires et/ou anti-oxydants contre les maladies neurodégénératives (Sclérose en Plaques, maladie d’Alzheimer…). Mise au point de systèmes supramoléculaires pour la manipulation et le dépôt de nanoparticules.

Publications

2011
 
41. “Tetrameric structure of SUR2B from electron microscopy of oriented single particles“
Constantina Fotinou, Jussio Aittoniemi, Heidi de Wet, Ange Polidori, Bernard Pucci, Mark S.P. Sansom, Catherine Venien-Bryan, Frances M. Ashcroft.
Biophys. J. (2011) Submitted

40. “Synthesis and aqueous self-aggregation studies of novel persubstitued benzene based facial surfactants. “
Julien Dauvergne, Anissa Bendjeriou, Françoise Bonneté, Ange Polidori and Bernard Pucci.
New J. Chem. (2011), submitted
 
39. “Synthesis and determination of polymerization rate constants of glucose-based monomers“
Shivaji K. Sharma, Grégory Durand  and Bernard Pucci
Designed Monomers and Polymers, (2011) in press

38. “New Glucose-Based Fluorinated Surfactants to Handle Integral Membrane Proteins in Aqueous Solution“
Maher Abla, Grégory Durand, Cécile Breyton,  Simon Raynal, Christine Ebel and Bernard Pucci.
Journal of Fluorine Chemistry (2011), in press

37. “Propyl-Ended Hemifluorinated Surfactants: Synthesis and Self-Assembling Properties“
Maher Abla, Grégory Durand and Bernard Pucci
Journal of Organic Chemistry (2011), 76, 2084-2093.

36. “Inhibitory potential of chemical substitutions at bioinspired sites of galactose on two classes of medically relevant lectins“
Denis Giguère, Sabine André, Marc-André Bonin, Marc-André Bellefleur, Alexandre Provencal, Philippe Cloutier, Bernard Pucci, René Roy, Hans-Joachim Gabius.
Bioorg. Med. Chem. (2011) 19, 3280-3287.

35. “Amphipols from A to Z“
Jean-Luc Popot, Thorsten Althoff, Dominique Bagnard, Jean-Louis Banères, Paola Bazzacco, Cherine Béchara, Emmanuelle Billon-Denis, Gérard Bolbach, Laurent Catoire, Philippe Champeil, Delphine Charvolin, Gérard Crémel, Tassadite Dahmane, Christine Ebel, Frank Gabel, Fabrice Giusti, Yann Gohon, Erik Goormaghtigh, Eric Guittet, Pascal Kessler, Jörg H. Kleinschmidt, Werner Kühlbrandt, Christel Le Bon, Karen L. Martinez, Alain Milon, Martin Picard, Bernard Pucci, Fabrice Rappaport, Marie Renault, Jonathan N. Sachs, Sandrine Sagan, Moeava Tehei, Christophe Tribet, Carine van Heijenoort, Frank Wien, Françoise Winnik, Giuseppe Zaccaï, Francesca Zito and Manuela Zoonens
Annu. Rev. Biochem, (2011) 40, 379-40.

34. “In the caul­dron of cell-free synthesis of membrane proteins: playing with new surfactants“
Kyu-Ho Park, Emmanuelle Billon-Denis, Tassadite Dahmane, Florence Lebaupain, Bernard Pucci, Cécile Breyton and Francesca Zito
New Biotechnology (2011), 28, 255-261

33. “A class of mild detergents for solubilisation that keeps integral membranes proteins water-soluble for functional studies and crystallisation“
Jens Hovers, Meike Potschies, Ange Polidori, Bernard Pucci, Simon Raynal, Maria Serrano-Vega, Chris Tate, Daniel Picot, Yves Pierre, Jean-Luc Popot, Rony Nehmé, Michel Bidet, Isabelle Mus-Veteau, Holger Bußkamp, Karl-Heinz Jung, Andreas Marx, P.A. Timmins, Wolfram Welte
Molecular Membrane Biology (2011) 28, 170-180

2010

32. “Cholesterol-Based alpha-Phenyl-N-tert-butyl nitrone derivatives as Antioxidants Against Light-Induced Retinal       Degeneration. “
Fanny Choteau, Grégory Durand, Isabelle Ranchon-Cole, Christine Cercy, Fernand Léal, and Bernard Pucci
Bioorg. Med. Chem. Lett. (2010), 20, 7405-78409.

31. “Amphiphilic Amide Nitrones: A New Class of Protective Agents Acting as Modifiers of Mitochondrial Metabolism“
Grégory Durand, Stéphanie Ortial, Burkhard Poeggeler, Ange Polidori, Frederick A. Villamena, Jutta Böker, Rüdiger Hardeland, Miguel. A. Pappolla and Bernard Pucci.
J. Med. Chem., 2010, 53 (13), pp 4849–4861.

30. “Synthesis, Physical-Chemical and Biological Properties of Amphiphilic Amino Acid-Based Nitroxides”
Grégory Durand, Fanny Choteau, Robert A. Prosak, Antal Rockenbauer, Frederick A. Villamena and Bernard Pucci.
New J. Chem. (2010), 34, 1909-1920.

29. “Novel thalidomide derivatives as candidates for Multiple Sclerosis treatment”
Christiane Contino-Pépin, Audrey Parat, Cindy Patinote, Stephen J. Karlik,  and Bernard Pucci.
Chem. Med. Chem, (2010) 5, 2057-2064.

28. “Stability study of the human G-protein coupled receptor, smoothened “
Rony Nehmé, Olivier Joubert, Michel Bidet, Benoît Lacombe, Ange Polidori, Bernard Pucci, Isabelle Mus-Veteau.
Biochimica et Biophysica Acta  (2010) 1798, 1100–1110

27. “Characterization of fish type III antifreeze protein in solution: structure and function“
Andrés G. Salvay, Frank Gabel, Bernard Pucci, Javier Santos, Eduardo I. Howard, Christine Ebel
Biophys. J.  (2010) 99, 609-618.

2009

26.  “Lipophilic β-Cyclodextrin Cyclic-Nitrone Conjugate: Synthesis and Spin Trapping Studies“
Han Y., Liu Y., Rockenbauer A., Zweier J.L., Durand G., Villamena F.A.
J. Org. Chem. (2009) 74, 5369-5380.

25. “Spin-trapping and Cytoprotective Properties of Fluorinated Amphiphilic Carrier Conjugates of Cyclic versus Linear Nitrones”
Grégory Durand, Robert A. Prosak, Yongbin Han,  Stéphanie Ortial, Antal Rockenbauer, Bernard Pucci, and Frederick A. Villamena,
Chem. Res. Toxicol.  (2009),  22,  1570–1581

24. “Trapping and stabilization of integral membrane proteins by hydrophobically grafted glucose-based telomers“
Paola Bazzacco, K. Shivaji Sharma, Grégory Durand, Fabrice Giusti, Christine Ebel, Jean-Luc Popot and Bernard Pucci.
Biomacromolecules (2009) 10, 3317-3326

23. “Amphiphilic perfluoroalkyl carbohydrates as new tools for liver imaging“
C. Richard, P. Chaumet-Riffaud, A. Belland, A. Parat, C. Contino-Pepin, M. Bessodes, D. Scherman, B. Pucci, N. Mignet
Inter. J. Pharmaceutics (2009), 379, 301-308.

22. “Hydrogenated and fluorinated surfactants derived from Tris(hydroxymethyl)-acrylamidomethane allow the purification of a highly active yeast F1F0 ATP synthase with an enhanced  stability".
 Talbot, Jean-Claude; Dautant, Alain; Polidori, Ange; Pucci, Bernard; Cohen-Bouhacina, Touria; Maali, Abdelhamid; Salin, Bénédicte; Brèthes, Daniel; Velours, Jean; Giraud, Marie-France
Journal of Bioenergetics and Biomembranes(2009) , 41, 349-360.

21. “Micellar and biochemical properties of (hemi)fluorinated surfactants are controlled by the size of the polar head”
Cécile Breyton, Frank Gabel, Maher Abla, Yves Pierre, Florence Lebaupain, Gregory Durand, Jean-Luc Popot, Christine Ebel and Bernard Pucci
Biophys. J. (2009), 97, 1077-1086.

20. “The human receptor Patched expressed in yeast interacts with its ligand Sonic Hedgehog both in membrane preparations and after purification“
Joubert Olivier, Nehme Rony, Fleury Damien, De Rivoyre Matthieu, Bidet Michel, Ruat Martial, Polidori Ange, Pucci Bernard, Mollat Patrick, Mus-Veteau Isabelle
BBA Biomembranes (2009), 1788, 1813–1821.

19. “Engineering a G protein-coupled receptor for structural studies: stabilization of the BLT1 receptor ground state.”
Martin, A., Damian, D., Laguerre, M., Parello, J., Pucci, B., Serre, L., Mary, S., Marie, J., and Banères, J.-L.
Protein Science  (2009) 18, 727-734.

18. “Preliminary biological evaluations of new thalidomide analogues for multiple sclerosis application.
Christiane Contino-Pépin, Audrey Parat, Sandrine Périno, Christine Lenoir, Michel Vidal, Hervé Galons, Stephen Karlik and Bernard Pucci.
Biorg Med Chem Lett. (2009) 19, 878–881.

17.“A Structure-Activity Investigation of Hemifluorinated Bifunctional Bolaamphiphiles Designed for Gene Delivery”
Brunelle, A. Polidori, S. Denoyelle, A.S. Fabiano, P.Y. Vuillaume, S. Laurent,  B. Pucci.
CR. Acad. Sci.(Special Gilles de Gennes) (2009), 12, 188-208.

2008

16. “FCS Study of Thermodynamics of Membrane Protein Insertion into Lipid Bilayer Chaperoned by Fluorinated Surfactants”
Yevgen O. Posokhov,  Mykola V. Rodnin, Bernard Pucci, and Alexey S. Ladokhin
Biophys J.-Biophys. Lett (2008), 8, L54-L56.

15. “Glucose-Based Surfactants with Hydrogenated, Fluorinated, or Hemifluorinated Tails: Synthesis and    Comparative Physical-Chemical Characterization”
Maher Abla, Gregory Durand, Bernard Pucci
J. Org. Chem. (2008), 73, 8142 – 8153

14. “Reactivity of superoxide radical anion and Hydroperoxyl radical with alpha-Phenyl-N-tert-butyl Nitrone (PBN) Derivatives“
Gregory Durand, Fanny Choteau, Bernard Pucci, Erick Villamena.
J. Phys. Chem. A. (2008), 112, 12498–12509

13. “Glucose-Based Amphiphilic Telomers Designed to Keep Membrane Proteins Soluble in Aqueous Solutions: Synthesis and Physical-Chemical Characterization”
K. Shivaji Sharma, Grégory Durand, Fabrice Giusti, Blandine Olivier, Anne-Sylvie Fabiano, Paola Bazzacco, Tassadite Dahmane, Christine Ebel, Jean-Luc Popot and Bernard Pucci
Langmuir (2008), 24, 13581-13590.

12. “Synthesis of a hemifluorinated amphiphile designed for self-assembly and two dimensional crystallization of  membrane proteins.”
Julien Dauvergne, Ange Polidori, Catherine Vénien-Bryan, Bernard Pucci.
Tet Lett. (2008) ,49, 2247-2250.

11. “Molecular transfer of surfactant bilayers: widening the range of the possible substrates”
Gaëlle Andreatta, Yong Jian Wang , Fuk Kay Lee, Ange Polidori, Penger Tong, Bernard Pucci and Jean-Jacques Benattar.
Langmuir (2008), 24, 6072-6078.

10. ”Interactions of fluorinated surfactants with diphtheria toxin t-domain: testing new media for studies of membrane proteins”
Mykola V. Rodnin, Yevgen O. Posokhov, Christiane Contino-Pépin, Joshua Brettmann, Alexander Kyrychenko, Sergiy Palchevskyy, Bernard Pucci, and Alexey S. Ladokhin.
Biophys J. (2008), 94, 4348-4357.
 
9. “Deposition of organized surfactant films on solid substrates”
F.  Andreatta, J.-J. Benattar, R. Petkova, J.Y.J. Wang, P. Tong, A. Polidori and B. Pucci
Colloids and Surfaces A: Physicochemical and Engineering Aspects (2008) 32, 211-217.

2007

8. ”Amphiphilic nitrone amides: A new class of protective agents acting as mitochondrial metabolism modifier “
Durand G, Poeggeler B, Ortial S, Polidori A, Boker J, Hardeland R, Pucci B
Free Rad. Bio. Med. (2007) 43, S91-S92. 

 7. “Fine-Tuning the Amphiphilicity: A Crucial Parameter in the Design of Potent a-Phenyl-N-tert-butylnitrone Analogs“
Grégory Durand, Burkhard Poeggeler,  Jutta Böker, Simon Raynal, Ange Polidori, Miguel A. Pappolla, Rüdiger Hardeland, and Bernard Pucci.
J. Med. Chem (2007) 50, 3976-3979.

6. “Study of b-Cyclodextrin/Fluorinated Trimethyl Ammonium Bromide Surfactant Inclusion Complex by Fluorinated Surfactant Ion Selective Electrode“
Sandeep R. Patil, Mireille Turmine, Véronique Peyre,Grégory  Durand, Bernard Pucci.
Talanta,( 2007), 74,72-77

5. “Stabilization of a Human GPCR in Solution by New Tensio-active Molecules“
Marjorie Damian, Sandrine Perino, Ange Polidori, Laurence Serre, Bernard Pucci and Jean-Louis Banères.
FEBS Letters, (2007), 581, 1944-1950.

4. “Mixtures of hydrogenated and fluorinated lactobionamide surfactants with cationic surfactants : study of     hydrogenated and fluorinated chains miscibility through potentiometric techniques”
Véronique Peyre, Sandeep Patil, Gregory Durand, Bernard Pucci.
Langmuir (2007), 23, 11465-11474.

3. “Thin freestanding films of fluorinated surfactants as a matrix for organizing detergent-solubilized membrane proteins in a plane”
Vera Petkova, Jean-Jacques Benattar, Manuela Zoonens, Francesca Zito, Jean-Luc Popot, Ange Polidori, Sylvain Jasseron and Bernard Pucci.
Langmuir (2007), 23, 4303-4309.

2. “A new amphiphilic derivative, LPBNSH, has a protective effect against copper-induced fulminant hepatitis in Long-Evans Cinnamon rats at an extremely low concentration compared with its original form a-phenyl-N-tert-butyl nitrone”
Taketoshi Asanuma, Hironobu Yasui, Osamu Inanami, Kenji Waki, Momoko Takahashi, Daisuke Iizuka, Taketo Uemura, Grégory Durand, Ange Polidori, Yasuhiro Kon, Bernard Pucci, Mikinori Kuwabara.
Chemistry & Biodiversity. (2007), 4, 2253-2267.

1. “Fluorinated and hemifluorinated surfactants as alternatives to classical detergents for membrane protein cell-free synthesis”
Kyu-Ho Park, Catherine Berrier, Florence Lebaupain, Bernard Pucci, Jean-Luc Popot, Alexandre Ghazi and Francesca Zito.
Biochem. J., (2007) 403,183-187.

Equipements

RMN 250 MHz multi-noyaux (Bruker)

Spectromètre de résonance paramagnétique électronique (Magnettech)

Spectromètres infra-rouge et UV/vis (Varian), spectrophotomètre (FluoroMax-2)

Tensiomètre (Kruss K 100), granulomètre (Malvern Nano S), balance de Langmuir

Electrophorèse capillaire (Iatroscan MK-5), polarimètre

CLHP analytique semi-préparative et préparative (Varian & Waters)

Combiflash

Chromatographie d’exclusion de taille (Waters et colonnes PSS)

Micro-onde, ultrasons (cuves Bransons et Bioblock VibraCell 75-043)

Lyophilisateur (BioBlock Alpha 1-4LD)

Voltampérométrie (VHS-JVC)

Centrifugeuse (ThermoScientific)

Coopérations internationales

Outils amphiphiles pour la protéomique
Dr Wolfram Welte, Fachbereich biologie, Universitat Konstanz, Konstanz, Germany.
Pr Alex Ladokhin, Kansas University, Medical center.
Pr Andrès Salvay, Universidad Nacional de la Plata, Argentine

Vectorisation d’agents anti-angiogéniques
Dr Stephen Karlik, MS researches department, Children’s Hospital, London, Ontario
Pr M. Touaibia, Laboratoire de chimie médicinale, Université de Moncton, Canada.
Pr Qian Peng, Department of Pathology, The Oslo University Hospital, Norway.
Pr. John McLarnon, Department of anaesthesiology, pharmacology and therapeutics. University of British Columbia, Vancouver – Canada
Pr. R. Roy, Laboratoire de Chimie Médicinale. Chaire de recherche du Canada. Université du Québec à Montréal (UQAM), Montréal – Canada
 
Nouveaux agents antiradicalaires
Pr F. Villamena, Davis Heart and Lung Research Institute, Ohio S. Univ, Columbus,
Pr Antal Rockenbauer, Université de Budapest, Hongrie.

Collaborations universitaires

Outils amphiphiles pour la protéomique
Jean-Luc Popot, Institut de Biologie Physico-Chimique, UMR 7099, Paris
Eva Pebay-Peyroula, LPM, IBS, Grenoble
Bruno Ameduri, Institut Charles Gerhardt - UMR(CNRS) 5253, Montpellier
Jean Claude Talbot, IBGC UMR 5095, Bordeaux
Corinne Vives, IBS, LPM, Grenoble
Bruno Miroux, Biotram UPR CNRS 9078, Paris.
Jean Jacques Lacapère, INSERM U773, CRB3, Paris
Colette Jungas, CNRS UMR 6161, IBEB/SBVMe-SBVMe/LBC, Cadarache
Olivier Joubert, CEA/DSV/IBITEC-S/SIMOPRO/LTMN CEA Gif/Yvette
Marie-France Giraud, Institut de Biochimie et Génétique Cellulaire UMR 5095, Bordeaux
Christine Ebel, IBS, LBM, Grenoble
Valentin Gordelyi, IBS, LBM, Grenoble
Cécile Breyton, IBS, LBM, Grenoble
Jean-Louis Baneres, IBMM, Montpellier
Laurence Serres, LPM, IBS, Grenoble.
Isabelle Mus-Veteau, FRE CNRS 3094, Nice Sophia Antipolis

Agents de contraste ultrasonore nanométriques pour l’imagerie et le traitement médical
Nicolas Taulier, Laboratoire d’imagerie paramétrique, UMR 8550, Université Paris 6.

Organisation et dépôt de nanoparticules en film noir
Jean-Jacques Benattar, CNRS URA 2464) DSM/DRECAM/SPEC, CEA Saclay.

Nouveaux agents antiradicalaires
Isabelle Ranchon-Cole, Faculté de Pharmacie, Clermont Ferrand

Extraction sans solvants d’ions par flottation
Pierre Bauduin, Institut de Chimie Séparative de Marcoule (ICSM) UMR 5257-LIIC (Laboratoire des Ions aux Interfaces Corrosives)
Olivier Diat, Institut de Chimie Séparative de Marcoule (ICSM) UMR 5257-LIIC (Laboratoire des Ions aux Interfaces Corrosives)

Nanoparticules métalliques pegylées, modélisation du cheminement des virus dans les sols
Eric Michel, UMR EMMAH, Centre INRA d’Avignon.
Sophie Neveu, laboratoire PECSA UMR 7195, Université Paris 6.